This manual will help you get to know your SMARTIO 2.5 software for your video tracking and laser optogenetic system.

SMARTIO is the specific evolution of the SMART 3.0 video-tracking software adapted for combining the video recording and video tracking functions of the SMART 3.0 with neural recording and stimulation (electrical and/or optogenetics). The version 2.5 includes several improvements:

• The possibility to record videos and from up to 16 image sources at the same time, thanks to the integration of SMARTIO 2.5 with Record-IT! Media 1.0.03.
• The possibility to acquire tracking data from up to 8 image sources at the same time.
• The possibility to integrate the tracking data with neural recording and stimulation (electrical and/or optogenetics) in up to 8 image sources at the same time.

Commercially, the SMARTIO functions are available through the SMARTIOBASIC package, including SMARTIO license and customized (CS), global activity (GA) and TriWise (TW) extensions, and the SMARTIOSUPER packages, including everything included in the SMARTIOBASIC package plus the Multiple Arenas (MA) extension.